Researchers from FHNW University of Applied Sciences and Arts Northwestern Switzerland explored a 23-day NIST CHO cell perfusion cultivation using the Multifors 3 bench-top bioreactor with the Multifors 3 Perfusion Package. The culture maintained viability consistently above 95% at a perfusion rate of 2 vvd. Multifors 3 controlled the bioreactor-side variables throughout the process, including gravimetric volume and feed control, pH, dissolved oxygen and biomass, with process parameters configured in eve and executed by Multifors 3.

Perfusion is becoming an increasingly important strategy for intensifying cell culture processes, but developing robust workflows requires precise process control and reliable laboratory-scale data. In this collaborative study from ZHAW Zurich University of Applied Sciences and Levitronix GmbH researchers demonstrate how ultra-high cell density ExpiCHO-S perfusion cultivations were achieved using the Minifors 2 bench-top bioreactor with integrated tangential flow filtration (TFF) and eve bioprocess software, providing practical insights for continuous bioprocess development and N-1 seed train applications.