Published: 13 sep. 2024(Updated: 13 sep. 2024)
•28 min
•28 min read
•Jeffrey L. Schloßhauer, Lena Tholen, Alexander Körner, Stefan Kubick, Sofia Chatzopoulou, Anja Hönow, Anne ZemellaPromoting the production of challenging proteins via induced expression in CHO cells and modified cell-free lysates harboring T7 RNA polymerase and mutant eIF2α
This study from Fraunhofer IZI-BB and Freie Universität Berlin introduces innovative strategies to boost biopharmaceutical production in CHO cells. By integrating mutant eIF2 and T7 RNA polymerase, researchers achieved higher yields in cell-free protein synthesis, offering flexibility between cell-based and cell-free methods. Explore how these advancements could revolutionize the production of challenging proteins.