A Flexible Hybrid Site‐Specific Integration‐Based Expression System in CHO Cells for Higher‐Throughput Evaluation of Monoclonal Antibody Expression Cassettes

Researchers from the University of Delaware's Department of Chemical and Biomolecular Engineering have developed a site-specific integration (SSI) system to streamline CHO cell line development for monoclonal antibody (mAb) production. Using the INFORS HT Multitron incubator shaker, they cultivated cells under optimized conditions to evaluate a recombinase-mediated cassette exchange (RMCE) system that enables high-throughput transgene selection without cell sorting. Their approach resulted in a 7- to 11-fold increase in mAb productivity, offering a faster and more reliable method for biopharmaceutical manufacturing.