Publikationen

Researchers from the Chair of Nutrition and Immunology, ZIEL - Institute for Food and Health, and the Data Science in Systems Biology group at the Technical University of Munich (Germany) investigated how diet influences the development of the infant gut microbiome during the first year of life. While age was found to be the primary driver of microbiota assembly, formula composition influenced metabolite profiles and the development of bacterial circadian rhythms. Using the INFORS HT Multifors 2 bioreactor as an ex vivo gut chemostat model, the team confirmed that rhythmic behavior in dominant gut bacteria can be maintained under controlled laboratory conditions, providing new insights into the relationship between nutrition, microbiome development, and circadian biology.

Researchers from the KTH Royal Institute of Technology (Sweden) used enzyme-constrained genome-scale models (ecGEMs) to redesign the anaerobic metabolism of Saccharomyces cerevisiae for the co-production of 2,3-butanediol and glycerol. Seed cultures were prepared using the INFORS HT Minitron incubator shaker before fermentation experiments, enabling validation of model predictions through bioreactor cultivation and proteomic analysis. The study demonstrated that engineered yeast cells could achieve high glucose uptake rates by reallocating cellular resources toward glycolysis, highlighting the potential of ecGEMs as a powerful tool for metabolic engineering and strain development.

Researchers from the Department of Chemical & Biomolecular Engineering and the Department of Electrical and Computer Engineering at the University of Delaware (USA) developed a transcriptomic workflow called MemorySeq to identify stress-responsive biomarkers in Chinese hamster ovary (CHO) cells exposed to manufacturing-related stress conditions. Using the INFORS HT Minitron incubator shaker for controlled CHO cell cultivation, the study identified 199 genes with heritable transcriptional variability linked to stress adaptation, apoptosis regulation, and metabolic pathways. These findings provide new insights into engineering more stress-tolerant CHO cell lines for improved biopharmaceutical manufacturing performance.

Researchers at the Institute of Environmental Biotechnology, BOKU University in Austria developed a sustainable method to recycle blended wool-polyester textile waste using enzymatic hydrolysis followed by microbial valorization. Using the INFORS HT Multitron Standard incubator shaker, they cultivated Chlorella vulgaris and Rhodotorula mucilaginosa on recovered wool hydrolysates, producing valuable bioproducts including natural pigments and lipids. This innovative process transforms textile waste into renewable resources for dyes and biofuels, supporting the circular bioeconomy.

This study demonstrates how vine shoot waste, an underutilized agricultural byproduct, can be transformed into fermentable sugars using choline chloride-based deep eutectic solvents (DESs). Researchers evaluated two DES formulations and found that ChCl:lactic acid (1:5) pretreatment significantly enhanced carbohydrate conversion, achieving rates of up to 75.2% for cellulose and 99.9% for xylan. Enzymatic hydrolysis of pretreated biomass was performed in the INFORS HT Minitron incubator shaker, enabling controlled saccharification. The results support a greener, efficient approach to biomass fractionation and lignin recovery, with strong potential for sustainable bioprocessing and agricultural waste valorization.

Researchers have sequenced and characterized a novel thermophilic cyanobacterium isolated from a hot spring in Namibia, revealing how it adapts to extreme heat through temperature responsive protein expression. Cultivated using the INFORS HT Labfors bench-top bioreactor, the strain proposed to represent a new genus within the Thermosynechococcaceae family displayed distinct genomic and proteomic features linked to thermotolerance. These findings expand our understanding of extremophile adaptation and highlight promising targets for future bioengineering and high temperature biotechnological applications.

In this application note, Enzyferm AS compares bioreactor and shaking flask systems for high-temperature aerobic propagation of Kveik yeast. Using the INFORS HT Minifors 2 and Multitron Standard, the study evaluates ease of use, process control, and reproducibility when working with single- and multi-strain cultures at. The results highlight how system choice impacts robustness and consistency in demanding fermentation conditions.

In collaboration with Regenera Moléculas do Mar and Ecovias Cerrado, this application note presents the successful scale-up of a bioluminescent marine strain, Aliivibrio sp. (ref. 1485), using the INFORS HT Minifors 2 bioreactor and eve bioprocess software. The study validated a reproducible cultivation protocol and examined the light-emission kinetics of the strain under controlled conditions. These findings contribute to a deeper understanding of marine bioprocesses and demonstrate how precise bioreactor control supports the development of bioluminescent applications.

Researchers have demonstrated how Bacillus subtilis BS20 can be optimized as a potent biocontrol agent for agriculture by scaling up antifungal metabolite production. Using the INFORS HT Labfors bench-top bioreactor, the team developed a process model and validated scale-up from 1 L to 10 L fermentation, maintaining high antifungal activity with a zone of inhibition up to 65 mm. This study offers a scalable, sustainable alternative to chemical pesticides through microbial fermentation.

Researchers at the University of Nottingham’s School of Biosciences have advanced vaccine development for Chagas disease by expressing and purifying a key enzyme target, TcPOP, in E. coli using the INFORS HT Techfors-S pilot bioreactor. The purified protein enabled structural and immunological characterization, showing parasite-neutralizing activity in mouse models and providing insights into the conformational dynamics of the enzyme. These findings offer a strong foundation for the development of a targeted, protein-based vaccine candidate for a globally neglected tropical disease.