Publications

This study demonstrates how vine shoot waste, an underutilized agricultural byproduct, can be transformed into fermentable sugars using choline chloride-based deep eutectic solvents (DESs). Researchers evaluated two DES formulations and found that ChCl:lactic acid (1:5) pretreatment significantly enhanced carbohydrate conversion, achieving rates of up to 75.2% for cellulose and 99.9% for xylan. Enzymatic hydrolysis of pretreated biomass was performed in the INFORS HT Minitron incubator shaker, enabling controlled saccharification. The results support a greener, efficient approach to biomass fractionation and lignin recovery, with strong potential for sustainable bioprocessing and agricultural waste valorization.

Researchers have sequenced and characterized a novel thermophilic cyanobacterium isolated from a hot spring in Namibia, revealing how it adapts to extreme heat through temperature responsive protein expression. Cultivated using the INFORS HT Labfors bench-top bioreactor, the strain proposed to represent a new genus within the Thermosynechococcaceae family displayed distinct genomic and proteomic features linked to thermotolerance. These findings expand our understanding of extremophile adaptation and highlight promising targets for future bioengineering and high temperature biotechnological applications.

This study done by BiOinFood, achieved a 50% increase in biomass and a 15% yield improvement by converting babassu flour into single-cell protein through fed-batch yeast cultivation using the INFORS HT Multifors bench-top bioreactor with eve software and the Minitron incubator shaker. With optimized process parameters and continuous feeding, the resulting protein-rich biomass was successfully used in plant-based burger prototypes, demonstrating a promising path for upcycling agricultural byproducts into functional food ingredients.

Researchers from Adamo Foods and the University of Nottingham investigated how nutritional manipulation of fermentation media, specifically the addition of iron (Fe III) and Vitamin B12, impacts the yield, texture, and nutritional profile of fungal mycelium grown for alternative meat applications. Using the INFORS HT Techfors-S pilot bioreactor, the team scaled up production from shake flasks and observed significant increases in bioaccumulation of iron and B12, with up to 97% terephthalic acid yield sustained across multiple cycles. Sensory testing of biomass processed into steaks also revealed changes in texture and flavor, highlighting the importance of bioreactor-based media optimization for developing high-quality, reproducible mycelium-based products.

In this application note, Enzyferm AS compares bioreactor and shaking flask systems for high-temperature aerobic propagation of Kveik yeast. Using the INFORS HT Minifors 2 and Multitron Standard, the study evaluates ease of use, process control, and reproducibility when working with single- and multi-strain cultures at. The results highlight how system choice impacts robustness and consistency in demanding fermentation conditions.

In collaboration with Regenera Moléculas do Mar and Ecovias Cerrado, this application note presents the successful scale-up of a bioluminescent marine strain, Aliivibrio sp. (ref. 1485), using the INFORS HT Minifors 2 bioreactor and eve bioprocess software. The study validated a reproducible cultivation protocol and examined the light-emission kinetics of the strain under controlled conditions. These findings contribute to a deeper understanding of marine bioprocesses and demonstrate how precise bioreactor control supports the development of bioluminescent applications.

Researchers have demonstrated how Bacillus subtilis BS20 can be optimized as a potent biocontrol agent for agriculture by scaling up antifungal metabolite production. Using the INFORS HT Labfors bench-top bioreactor, the team developed a process model and validated scale-up from 1 L to 10 L fermentation, maintaining high antifungal activity with a zone of inhibition up to 65 mm. This study offers a scalable, sustainable alternative to chemical pesticides through microbial fermentation.

Researchers at the University of Nottingham’s School of Biosciences have advanced vaccine development for Chagas disease by expressing and purifying a key enzyme target, TcPOP, in E. coli using the INFORS HT Techfors-S pilot bioreactor. The purified protein enabled structural and immunological characterization, showing parasite-neutralizing activity in mouse models and providing insights into the conformational dynamics of the enzyme. These findings offer a strong foundation for the development of a targeted, protein-based vaccine candidate for a globally neglected tropical disease.

At the University of Boras in Sweden, researchers have scaled up second-generation bioethanol production from beech wood chips using an acetone-based organosolv fractionation process. With the help of the INFORS HT Multifors bench-top bioreactor, they confirmed high fermentation efficiency at the 10-L scale, reaching ethanol yields of up to 95% from glucose-rich C6 streams. This study illustrates how optimized biomass pre-treatment and fermentation workflows can drive more efficient and scalable biofuel production.

At Lund University, Division of Food and Pharma, researchers studied the role of annealing in the freeze-drying of probiotic bacteria. Using the INFORS HT Multifors bioreactor and eve software, they controlled the fermentation of Limosilactobacillus reuteri to produce consistent cell cultures for downstream drying. Their results show that increased annealing time leads to thicker encapsulating structures and enhanced storage stability, providing a clearer path to developing more robust probiotic products.