Researchers from the Institute of Bioprocess Engineering and Pharmaceutical Technology at the University of Applied Sciences Mittelhessen have made strides in chemoorganotrophic electrofermentation using Cupriavidus necator. By experimenting with different redox mediators, they identified ferricyanide as a key player in enhancing anodic respiration. With the INFORS HT Multitron providing precise environmental control, the team achieved remarkable current densities, offering a promising solution to the challenges of oxygen-driven fermentation systems.

Researchers from the University of Delaware, Departments of Chemical and Biomolecular Engineering and Electrical and Computer Engineering have made strides in enhancing the resilience of Chinese hamster ovary (CHO) cells used in biopharmaceutical production. By employing the INFORS HT Multitron incubator shaker, they exposed CHO cells to stress conditions commonly encountered during manufacturing, such as elevated levels of ammonia, lactate, and osmolality. Through comprehensive transcriptomic analysis, the team identified 199 genes exhibiting bistable expression, with seven emerging as prime candidates for engineering stress-resistant cell lines. This research holds promise for optimizing cell health and boosting productivity in large-scale bioreactor operations.

Contamination in incubator shakers can lead to compromised cultures, experimental setbacks, and costly operational downtime. Ensuring a sterile environment is important for maintaining reliable and reproducible results. In this fifth installment of our Back to Basics series, we explore the best practices for preventing contamination in your incubator shaker and highlight how the new HEPA filtration feature in the Multitron Incubator Shaker provides active and continuous protection.

In bioprocessing, selecting the right shaker parameters is essential for optimizing the growth and productivity of various organisms, including bacteria, yeast, and mammalian cells. By fine-tuning these parameters, scientists can create ideal environments for cultivation, maximizing process efficiency and reproducibility. In this installment of our Back to Basics blog series, we focus on how INFORS HT incubator shakers enable better control and flexibility to meet diverse cultivation needs.