NIST CHO Cell Perfusion Cultivation Using the Multifors 3 Bioreactor

Researchers from FHNW University of Applied Sciences and Arts Northwestern Switzerland explored a 23-day NIST CHO cell perfusion cultivation using the Multifors 3 bench-top bioreactor with the Multifors 3 Perfusion Package. The culture maintained viability consistently above 95% at a perfusion rate of 2 vvd. Multifors 3 controlled the bioreactor-side variables throughout the process, including gravimetric volume and feed control, pH, dissolved oxygen and biomass, with process parameters configured in eve and executed by Multifors 3.