Topic
Product
Selecting the correct orbital throw for your incubator shaker is critical for achieving optimal results in cell culture, bacterial growth, and other bioprocessing applications. In this installment of the Back to Basics series, we will explore what orbital throw is, why it matters, and how to make the best choice for your experiments.
Selecting the correct orbital throw for your incubator shaker is critical for achieving optimal results in cell culture, bacterial growth, and other bioprocessing applications. In this installment of the Back to Basics series, we will explore what orbital throw is, why it matters, and how to make the best choice for your experiments.
The idea of using our own cells to produce antibodies essential for survival no longer belongs to science fiction. Although it has been the focus of researchers for decades, the direct use of messenger RNA encoding antibodies has received recent market approval due to the urgency of the COVID-19 pandemic. As such, this platform technology is revolutionizing the way we think about biopharmaceutical production and has great potential for further applications. In the following article, you will learn about the key aspects of the application, production and use of mRNA, the software of life.
As the workhorses of life, proteins fulfil a variety of functions in our bodies. If their functionality is limited or missing, diseases can arise. Therefore, proteins and their production are a central key to research. Whether protein expression is to be controlled for experimental, biotechnological, or medical applications, the use of cells is often mandatory. Many researchers repeatedly face the same challenge of producing complex target protein fast and cheap. Transient transfection of recombinant plasmids (rpDNA) in mammalian cells is a popular method for this purpose. In the following article, we look at the success story of research assistant Damian von Blarer, who must produce specific regions of the famous SARS-CoV-2 spike protein for efficiency studies of a rapid test – for this he needs plasmids, and we show which questions you need to answer to produce and amplify them easily yourself.
In recent years, mRNA (messenger RNA) technology has emerged as a groundbreaking tool in the field of medicine, revolutionizing the way we approach disease prevention and treatment. This cutting-edge technology holds tremendous promise for combating a wide range of diseases, including infectious diseases, cancer and genetic disorders.
A plasmid DNA production process was performed comparing conventional Erlenmeyer flasks with LB medium and the Thomson’s Ultra Yield® system. E. coli cells were cultivated in both systems in the INFORS HT Multitron incubator shaker, bacterial growth was monitored, and the plasmids were purified at the end of cultivation. Subsequently, the quality of the isolated plasmids was analyzed by HPLC. The combination of the Ultra Yield® flasks with the enriched Plasmid+® medium and the AirOtop® enhanced seal led to higher cell densities and a 21-fold higher amount of plasmid than in Erlenmeyer shake flasks with LB medium.
Maintaining a sterile environment is essential in bioprocess laboratories, particularly when working with sensitive cell cultures or microbial samples. Contamination not only jeopardizes experimental results, but it can also lead to costly downtime and wasted resources. In this first installment of our Back to Basics blog series, we focus on the role of UV decontamination in incubator shakers and how it serves as an effective risk mitigation strategy.
As demand for sustainable food sources surges, Daisy Lab is revolutionizing dairy production with precision fermentation technology. By using INFORS HT bioreactors, this New Zealand-based startup is producing animal-free milk proteins like whey and casein. Discover how the innovative process at Daisy Labs, now set to scale with EPA approval, is paving the way for a more sustainable future in dairy.
Stefan Sperling is an industrial designer at the Meyer-Hayoz Design Engineering Group in Switzerland. He is part of the specialist team who was responsible for designing the award-winning INFORS HT Minifors 2 as well as the Multitron and Minitron. In this interview, Mr. Sperling shares his view on what constitutes good product design.
In the fast-paced world of bioprocessing, having reliable lab equipment is critical for research and process development success. The Multitron incubator shaker has been designed with these demands in mind, offering precision and efficiency that directly address the challenges scientists face in their labs. Whether you are optimizing culture conditions or scaling up processes, the Multitron shaker provides the tools you need to advance your research with confidence.
Gene therapy is a promising approach for treating various genetic disorders and diseases. A critical component of gene therapy is the production of viral vectors, which serve as delivery vehicles for therapeutic genes. Human Embryonic Kidney 293 (HEK293) cells have become a widely used platform for viral vector production due to their efficiency in transfection and ability to support viral replication. However, optimizing HEK293 cell cultures for large-scale production of viral vectors remains a challenge in making gene therapies more accessible and cost-effective.
The idea of using our own cells to produce antibodies essential for survival no longer belongs to science fiction. Although it has been the focus of researchers for decades, the direct use of messenger RNA encoding antibodies has received recent market approval due to the urgency of the COVID-19 pandemic. As such, this platform technology is revolutionizing the way we think about biopharmaceutical production and has great potential for further applications. In the following article, you will learn about the key aspects of the application, production and use of mRNA, the software of life.
As the workhorses of life, proteins fulfil a variety of functions in our bodies. If their functionality is limited or missing, diseases can arise. Therefore, proteins and their production are a central key to research. Whether protein expression is to be controlled for experimental, biotechnological, or medical applications, the use of cells is often mandatory. Many researchers repeatedly face the same challenge of producing complex target protein fast and cheap. Transient transfection of recombinant plasmids (rpDNA) in mammalian cells is a popular method for this purpose. In the following article, we look at the success story of research assistant Damian von Blarer, who must produce specific regions of the famous SARS-CoV-2 spike protein for efficiency studies of a rapid test – for this he needs plasmids, and we show which questions you need to answer to produce and amplify them easily yourself.
In recent years, mRNA (messenger RNA) technology has emerged as a groundbreaking tool in the field of medicine, revolutionizing the way we approach disease prevention and treatment. This cutting-edge technology holds tremendous promise for combating a wide range of diseases, including infectious diseases, cancer and genetic disorders.
A plasmid DNA production process was performed comparing conventional Erlenmeyer flasks with LB medium and the Thomson’s Ultra Yield® system. E. coli cells were cultivated in both systems in the INFORS HT Multitron incubator shaker, bacterial growth was monitored, and the plasmids were purified at the end of cultivation. Subsequently, the quality of the isolated plasmids was analyzed by HPLC. The combination of the Ultra Yield® flasks with the enriched Plasmid+® medium and the AirOtop® enhanced seal led to higher cell densities and a 21-fold higher amount of plasmid than in Erlenmeyer shake flasks with LB medium.
Maintaining a sterile environment is essential in bioprocess laboratories, particularly when working with sensitive cell cultures or microbial samples. Contamination not only jeopardizes experimental results, but it can also lead to costly downtime and wasted resources. In this first installment of our Back to Basics blog series, we focus on the role of UV decontamination in incubator shakers and how it serves as an effective risk mitigation strategy.
As demand for sustainable food sources surges, Daisy Lab is revolutionizing dairy production with precision fermentation technology. By using INFORS HT bioreactors, this New Zealand-based startup is producing animal-free milk proteins like whey and casein. Discover how the innovative process at Daisy Labs, now set to scale with EPA approval, is paving the way for a more sustainable future in dairy.
Stefan Sperling is an industrial designer at the Meyer-Hayoz Design Engineering Group in Switzerland. He is part of the specialist team who was responsible for designing the award-winning INFORS HT Minifors 2 as well as the Multitron and Minitron. In this interview, Mr. Sperling shares his view on what constitutes good product design.
In the fast-paced world of bioprocessing, having reliable lab equipment is critical for research and process development success. The Multitron incubator shaker has been designed with these demands in mind, offering precision and efficiency that directly address the challenges scientists face in their labs. Whether you are optimizing culture conditions or scaling up processes, the Multitron shaker provides the tools you need to advance your research with confidence.
Gene therapy is a promising approach for treating various genetic disorders and diseases. A critical component of gene therapy is the production of viral vectors, which serve as delivery vehicles for therapeutic genes. Human Embryonic Kidney 293 (HEK293) cells have become a widely used platform for viral vector production due to their efficiency in transfection and ability to support viral replication. However, optimizing HEK293 cell cultures for large-scale production of viral vectors remains a challenge in making gene therapies more accessible and cost-effective.