Publicações

Researchers from the University of Delaware, Departments of Chemical and Biomolecular Engineering and Electrical and Computer Engineering have made strides in enhancing the resilience of Chinese hamster ovary (CHO) cells used in biopharmaceutical production. By employing the INFORS HT Multitron incubator shaker, they exposed CHO cells to stress conditions commonly encountered during manufacturing, such as elevated levels of ammonia, lactate, and osmolality. Through comprehensive transcriptomic analysis, the team identified 199 genes exhibiting bistable expression, with seven emerging as prime candidates for engineering stress-resistant cell lines. This research holds promise for optimizing cell health and boosting productivity in large-scale bioreactor operations.

Researchers from the University of Delaware's Department of Chemical and Biomolecular Engineering have developed a site-specific integration (SSI) system to streamline CHO cell line development for monoclonal antibody (mAb) production. Using the INFORS HT Multitron incubator shaker, they cultivated cells under optimized conditions to evaluate a recombinase-mediated cassette exchange (RMCE) system that enables high-throughput transgene selection without cell sorting. Their approach resulted in a 7- to 11-fold increase in mAb productivity, offering a faster and more reliable method for biopharmaceutical manufacturing.

Researchers from Albert Einstein College of Medicine, Adimab LLC, and the U.S. Army Medical Research Institute of Infectious Diseases explored how combining neutralizing antibodies targeting different parts of the SARS-CoV-2 spike protein can help prevent escape mutants. Using the INFORS HT Multitron incubator shaker, they cultivated cells under controlled conditions to test antibody effectiveness. Their approach could strengthen antibody-based COVID-19 treatments by improving effectiveness and reducing resistance.

Scientists from the University of California, Berkeley, and The Rockefeller University have developed an innovative CRISPR-Cas9-integrase complex that enables precise DNA fragment integration, marking a significant leap forward in genome editing technology. The team employed the INFORS HT Multitron incubator shaker to maintain optimal cultivation conditions, ensuring the reliability of their experimental results. This approach is poised to enhance the precision and efficiency of genetic modifications, opening new avenues in biotechnology and therapeutic development.

Researchers at the University of Guelph have engineered the Robogut, a bioreactor model that emulates the human colon environment. Employing the Multifors bench-top bioreactor, this innovative system allows for precise cultivation and analysis of gut microbial communities, providing valuable insights into their roles in human health and disease.

How do microorganisms adapt to nutrient scarcity? Using the Multitron incubator shaker, researchers at Imperial College London studied how Saccharomyces cerevisiae responds to nitrogen downshifts. They found that nitrogen sources drive two distinct subpopulations: one focused on growth, the other on survival. This study reveals how amino acids act as key signaling molecules, shaping cellular behavior and adaptation.

How well does your body access curcuminoids from food? Researchers from Université de Technologie de Compiègne used the Multitron incubator shaker to study how fiber-enriched biscuits and custards affect curcuminoid bioaccessibility. Their Bayesian model revealed how food matrices impact nutrient absorption, offering insights to design healthier, optimized functional foods.

Researchers at TU Dortmund University have uncovered metabolic bottlenecks in Pseudomonas taiwanensis during growth on d-xylose, using the INFORS HT Multitron Standard incubator shaker. Their findings offer new insights into optimizing the Weimberg pathway for bioprocesses on renewable feedstocks, advancing strain development and bioproduction.

Researchers at the Helmholtz Centre for Environmental Research, in Germany, have developed an in vitro model to investigate how environmental chemicals, such as bisphenols (BPX) and PFAS mixtures, affect the interactions between the microbiome and immune system. Using the Multifors bench-top bioreactor, they demonstrated that chronic chemical exposure can alter immune cell activation without affecting microbial community structure.

In a study from the University of Aveiro, researchers leveraged the INFORS HT Minifors bench-top bioreactor to optimize recombinant laccase production in Komagataella phaffii. By fine-tuning cultivation conditions, they scaled laccase production and demonstrated its stability and effectiveness as a biocatalyst. Notably, this laccase was used to assist dopamine polymerization, achieving an innovative polydopamine coating on filter paper, an exciting advance in enzyme applications for material science.