Identifying stress-resistance biomarkers in CHO cells

Researchers from the Department of Chemical & Biomolecular Engineering and the Department of Electrical and Computer Engineering at the University of Delaware (USA) developed a transcriptomic workflow called MemorySeq to identify stress-responsive biomarkers in Chinese hamster ovary (CHO) cells exposed to manufacturing-related stress conditions. Using the INFORS HT Minitron incubator shaker for controlled CHO cell cultivation, the study identified 199 genes with heritable transcriptional variability linked to stress adaptation, apoptosis regulation, and metabolic pathways. These findings provide new insights into engineering more stress-tolerant CHO cell lines for improved biopharmaceutical manufacturing performance.