Perfusion Cultivations with ExpiCHO-S Cells Using the Minifors 2 Bioreactor

Perfusion is becoming an increasingly important strategy for intensifying cell culture processes, but developing robust workflows requires precise process control and reliable laboratory-scale data. In this collaborative study from ZHAW Zurich University of Applied Sciences and Levitronix GmbH researchers demonstrate how ultra-high cell density ExpiCHO-S perfusion cultivations were achieved using the Minifors 2 bench-top bioreactor with integrated tangential flow filtration (TFF) and eve bioprocess software, providing practical insights for continuous bioprocess development and N-1 seed train applications.